pEgr-TNFα基因联合放疗抑制食管癌细胞生长的实验研究

doi:10.3969/j.issn.1004-616x.2006.01.002

癌变·畸变·突变 ›› 2006, Vol. 18 ›› Issue (1): 6-008.doi: 10.3969/j.issn.1004-616x.2006.01.002

pEgr-TNFα基因联合放疗抑制食管癌细胞生长的实验研究

吴丛梅1;黄天华1;吴德生1;谢庆东1;李德锐2;陈秀如2;徐小虎3

1.汕头大学医学院生殖医学研究中心，广东　汕头　515041；2．汕头大学医学院肿瘤医院，广东　汕头　515041；3．汕头大学法医学教研室，广东　汕头　515041

收稿日期:2004-09-15 修回日期:2004-11-05 出版日期:2006-01-30 发布日期:2006-01-30
通讯作者: 黄天华

Experimental Study on Inhibition of pEgr-TNFα Gene Therapy Combined with Radiotherapy on Esophageal Cancer Cells

WU Cong-mei1,HUANG Tian-hua1,WU De-sheng1,XIE Qing-dong1,LI De-rui2,CHEN Xiu-ru2,XU Xiao-hu3

1.Research Center of Reproductive Medicine, Shantou University Medical College, Shantou 515041,Guangdong, China; 2. Tumor Hospital, Shantou University Medical College, Shantou 515041,Guangdong,China; 3. Forensic Department, Shantou University Medical College, Shantou 515041,Guangdong,China

Received:2004-09-15 Revised:2004-11-05 Online:2006-01-30 Published:2006-01-30
Contact: HUANG Tian-hua

摘要/Abstract

摘要： 背景与目的：研究pEgr-TNFα重组质粒在稳定转染的食管癌细胞中的辐射诱导表达，及其联合放射治疗抑制食管癌细胞生长的效果。材料与方法：将脂质体包裹的pEgr-TNFα重组质粒，转染食管癌细胞系EC9706中，经G418筛选，获得稳定表达的细胞；采用ELISA方法检测0.075 Gy和2 Gy剂量X射线诱导后TNFα的表达；观察该工种剂量X射线照射后，EC9706细胞的增长情况。结果： pEgr-TNFα重组质粒转染EC9706细胞，并获得稳定转染的细胞；大剂量X射线照射和低剂量X射线照射均可诱导TNFα表达增强，为对照组的6～6.3倍(P<0.01)；稳定转染的细胞经0.075 Gy和2 Gy X射线照射，8 d后细胞数明显低于未转染细胞组(P<0.01)和稳定转染的假照射组(P<0.01)。结论：体外pEgr-TNFα基因-放射联合治疗有明显的抑制食管癌细胞生长的作用。

关键词: pEgr-TNFα 质粒, X射线照射, 食管癌细胞, 低剂量照射

Abstract: BACKGROUND ＆ AIM: To study the expression of pEgr-TNFα induced by ionizing irradiation in stably transfected esophageal cancer cells and to study the inhibition effects of pEgr-TNFα combined with ionizing irradiation on esophageal cancer cells. MATERIAL AND METHODS: Esophageal cancer cell line EC9706 was transfected with pEgr-TNFα plasmids and selected through G418 to get stable expression cells. TNFα expression was detected by ELISA. The inhibition effect of pEgr-TNFα was observed after different doses of X-ray irradiation. RESULTS: pEgr-TNFα plasmids were transfected into EC9706 cells with stable expression. High dose and low dose X-ray irradiation could all enhance the expression of TNFα, about 6－6.3 times of control group (P<0.001). Cells stably transfected was given 0.075 Gy and 2 Gy X-ray irradiation, number of cells was less than non-transfected group and sham group (P<0.01～0.001) 8 d later. CONCLUSION: pEgr-TNFα gene-radiotherapy can significantly inhibit the growth of esophageal cancer cells in vitro.

Key words: pEgr-TNFα plasmid;X-ray irradiation, esophageal cancer cell;low dose irradiation

中图分类号:

R146

吴丛梅, 黄天华, 吴德生, 谢庆东, 李德锐, 陈秀如, 徐小虎. pEgr-TNFα基因联合放疗抑制食管癌细胞生长的实验研究[J]. 癌变·畸变·突变, 2006, 18(1): 6-008.

WU Cong-mei, HUANG Tian-hua, WU De-sheng, XIE Qing-dong, LI De-rui, CHEN Xiu-ru, XU Xiao-hu. Experimental Study on Inhibition of pEgr-TNFα Gene Therapy Combined with Radiotherapy on Esophageal Cancer Cells[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2006, 18(1): 6-008.

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pEgr-TNFα基因联合放疗抑制食管癌细胞生长的实验研究

Experimental Study on Inhibition of pEgr-TNFα Gene Therapy Combined with Radiotherapy on Esophageal Cancer Cells

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